专利名称：Method of adjusting the working range of a
multi-analyte assay
发明人：Roger Dowd,Jeffrey G. Donahue申请号：US11221198申请日：20050906
公开号：US20060177873A1公开日：20060810
摘要：The invention features a method of adjusting the concentration of at least onebut not all of a plurality of analytes in a fluid sample to match a known working range ofdetection of an analyte assay system, where each of the plurality of analytes may or maynot be present within an expected initial concentration range having a high end and a lowend, and at least one analyte has a high end expected concentration range that exceedsthe high end of the working range of the assay system. The expected concentration ofthe high concentration analyte is adjusted by a proportional scaling constant, α, so thatthe high end of the adjusted expected concentration range is less than or equal to thehigh end of the working range, without adjusting the expected concentration range of atleast one other of the plurality of analytes. Adjustment is preferably accomplished byadding to the solution phase of the assay one or more scaling agents, each scaling agentbinding with specificity to an analyte and thereby preventing it from being detected bythe assay system, e.g., by competing with binding to immobilized capture agent. Thisscaling method contrasts with prior methods, in which a concentration of availableanalyte is offset by a fixed amount to adjust the detectable threshold of the assay. Here,the amount of scaling agent is proportional to a scaling coefficient, and the scaling agent
is present in the solution phase of the assay at high concentrations relative to analyte.Due to the equilibrium conditions established by the laws of mass transfer, the amount offree analyte remaining in solution in the presence of scaling agent is predictable andfinite, and can be measured as a quantitative indicator of the initial concentration of theanalyte in the sample.
申请人：Roger Dowd,Jeffrey G. Donahue
地址：Natick MA US,Hopkinton MA US
国籍：US,US
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