专利名称：Method for the isolation of biological
macromolecules and apparatus forconducting said method
发明人：ZINN, THOMAS, DR.,MOELLER, KLAUS,
DR.,RADMACHER, EDMUND, DR.
申请号：EP03018362.8申请日：20030813公开号：EP1394269B1公开日：20050302
摘要：Microtitration plates (14, 23) respectively including first parallel- and secondcavities (16, 25) are arranged in apparatus (1, 41, 51) such that a first and a second cavity(16, 25) lie in succession. Filter units (20) are located in the first cavities (16),chromatographic material (29) in the second. The lysate is introduced into the firstcavities. Solution leaving the first cavities is transferred directly into the second cavities,the macromolecules being adsorbed onto the chromatographic material. The endotoxinsand other impurities are washed out. An elution fluid is introduced into the secondcavities, so that mixed fluid and macromolecules leaves the second microtitration plate.The mixture is transferred to a third microtitration plate (32) with parallel third cavities(34). Ultrafiltration units (35) are located in the third cavities. The third cavities aresubjected to a pressure difference or centrifugal force, acting towards their outletopenings. The macromolecules are recovered as retentate. The chromatographicmaterial is equilibrated with a buffer, before solution delivery. After lysate addition tothe first cavities, they are subjected to pressure difference or centrifugal force, acting
towards the second cavities. This is supplemented by corresponding action on the secondcavities. Further details of positioning and manipulation are elaborated. The agent usedfor washing is a nonionic tenside. This can be associated with an insert, a glass sinter orchromatographic material introduced. The filter units (20) are multistaged. Thechromatographic material is an anion exchange material. It is located between twosinters; it is a monolithic block or a membrane. The washing agent impregnating thesinters, enriches endotoxins. The chromatographic material contains a washing agentenriching endotoxins. An Independent claim is included for corresponding apparatus.
申请人：MACHEREY, NAGEL GMBH & CO. HANDELSGESELLSCHAFT,MACHEREY NAGELGMBH & CO HG,MACHEREY, NAGEL GMBH & CO. HANDELSGESELLSCHAFT
地址：DE
国籍：DE
代理机构：Paul, Dieter-Alfred, Dipl.-Ing.
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